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8x sypro orange dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 8x sypro orange dye
    8x Sypro Orange Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sypro+orange/Triton+X-100/pm42336208-485-35-39
    Average 99 stars, based on 1 article reviews
    8x sypro orange dye - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Development of a Novel Engineered Antibody Format for PSMA-Targeted Radionuclide Therapy.
    Article Snippet: Prostate cancer remains a prevalent and lethal malignancy across the globe.. Despite ongoing advances in therapeutic approaches, these remain ineffective, and new treatments are drastically needed.. Prostate-specific membrane antigen (PSMA)-targeted radionuclide therapy is a well-developed approach for prostate cancer treatment; however, current small molecule and antibody carriers for molecular radiotherapy each have drawbacks in their biodistribution and consequent side effects as highlighted in current clinical trials.

    Article Title: Structural Adaptations of Bacterial Grx3 to Temperature: Pro29 Is Essential for Cold Adaptation in Sphingomonas sp. Grx3
    Article Snippet: .. A thermal shift assay was performed using SYPRO Orange on an Applied Biosystems StepOnePlus real-time PCR instrument (Thermo Fisher Scientific, Waltham, MA, USA) with a continuous heating ramp from 25 to 99 °C at 1% per sec. SpGrx3 and EcGrx3 were added at a final concentration of 4 mg/mL to buffer D, with 25× and 250× SYPRO Orange, respectively, in a total volume of 20 μL. .. The melting temperature ( T m ), indicating the temperature at which 50% of the protein is denatured, was determined using Protein Thermal Shift software v1.4 (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Development of a Novel Engineered Antibody Format for PSMA-Targeted Radionuclide Therapy.
    Article Snippet: Prostate cancer remains a prevalent and lethal malignancy across the globe.. Despite ongoing advances in therapeutic approaches, these remain ineffective, and new treatments are drastically needed.. Prostate-specific membrane antigen (PSMA)-targeted radionuclide therapy is a well-developed approach for prostate cancer treatment; however, current small molecule and antibody carriers for molecular radiotherapy each have drawbacks in their biodistribution and consequent side effects as highlighted in current clinical trials.

    Article Title: Structural Adaptations of Bacterial Grx3 to Temperature: Pro29 Is Essential for Cold Adaptation in Sphingomonas sp. Grx3
    Article Snippet: .. A thermal shift assay was performed using SYPRO Orange on an Applied Biosystems StepOnePlus real-time PCR instrument (Thermo Fisher Scientific, Waltham, MA, USA) with a continuous heating ramp from 25 to 99 °C at 1% per sec. SpGrx3 and EcGrx3 were added at a final concentration of 4 mg/mL to buffer D, with 25× and 250× SYPRO Orange, respectively, in a total volume of 20 μL. .. The melting temperature ( T m ), indicating the temperature at which 50% of the protein is denatured, was determined using Protein Thermal Shift software v1.4 (Applied Biosystems).

    Article Title: Anti-cMet antibody-drug conjugates and uses thereof
    Article Snippet: The samples were added to 96-well PCR plates (Thermo Scientific Cat. No. AB-2400/W) and sealed with optical covers (Thermo Scientific Cat. No. 4360954). .. The PCR plate was inserted into a real-time PCR machine (Thermo Scientific Quant Studio 3) and the plate temperature was stabilized for 3 minutes at 25° C. before ramping to 95° C. by 0.2° C. increments, stabilizing for 1 second before the Sypro Orange signal was measured. ..

    Purification:

    Article Title: The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells.
    Article Snippet: .. Purified NIT2-WT (Cfinal 5 μM) or NIT2-C146A (Cfinal 5 μM), Triton X-100 (0.001% w/v), and SYPRO Orange (ThermoFisher Scientific) (2.5×) were mixed in phosphate buffer with or without DTT (100 mM KPO4, 5 mM DTT). ..

    Incubation:

    Article Title: Discovery of the First Highly Selective 1,4-dihydropyrido[3,4- b ]pyrazin-3(2H)-one MKK4 Inhibitor.
    Article Snippet: .. MKK4 protein was incubated with DMSO or 10 μM MKK4 inhibitor diluted from a 250 μM stock solution and 2.5x SYPRO Orange (Thermo Fischer) in TSA buffer containing 100 mM HEPES, pH 7.5 and 150 mM NaCl up to an end volume of 25 μL. ..

    Thermal Shift Assay:

    Article Title: Structural Adaptations of Bacterial Grx3 to Temperature: Pro29 Is Essential for Cold Adaptation in Sphingomonas sp. Grx3
    Article Snippet: .. A thermal shift assay was performed using SYPRO Orange on an Applied Biosystems StepOnePlus real-time PCR instrument (Thermo Fisher Scientific, Waltham, MA, USA) with a continuous heating ramp from 25 to 99 °C at 1% per sec. SpGrx3 and EcGrx3 were added at a final concentration of 4 mg/mL to buffer D, with 25× and 250× SYPRO Orange, respectively, in a total volume of 20 μL. .. The melting temperature ( T m ), indicating the temperature at which 50% of the protein is denatured, was determined using Protein Thermal Shift software v1.4 (Applied Biosystems).

    Stability Assay:

    Article Title: FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma.
    Article Snippet: Running buffer was histidine buffer (20mM histidine: Sigma-Aldrich, cat. 84088; 150mM NaCl: Sigma-Aldrich, cat. S5886; 0.05% Tween 20: Sigma-Aldrich, cat. P1379; pH=6.0) and recovery buffer was 20mM Tris-HCl (Sigma-Adlrich, cat. 10812846001) pH= 7.5 with 150mM NaCl (Sigma-Aldrich, cat. S5886)0.05%Tween20 (Sigma-Aldrich, cat. P1379). .. The thermal stability assay was performed using SYPRO Orange (Thermo Fisher Scientific, cat. S6650). .. Four microgram of protein at a concentration of 0.4 μg/μL per wasmixed with SYPROOrange solution diluted in PBS or artificial CSF (aCSF; 125mMNaCl: Sigma-Aldrich, cat. S5886; 26mM NaHCO3: Sigma-Aldrich, S6014; 1.25mM NaH2PO3, Sigma-Aldrich, cat. 04273; and 2.5mM KCl, Sigma-Aldrich, cat. P5405) and measured on a thermocycler (Bio-Rad) set to 2min at 20 °C, followed by a 0.2 °C increase/30 s until 95 °C.

    Polymerase Chain Reaction:

    Article Title: Anti-cMet antibody-drug conjugates and uses thereof
    Article Snippet: The samples were added to 96-well PCR plates (Thermo Scientific Cat. No. AB-2400/W) and sealed with optical covers (Thermo Scientific Cat. No. 4360954). .. The PCR plate was inserted into a real-time PCR machine (Thermo Scientific Quant Studio 3) and the plate temperature was stabilized for 3 minutes at 25° C. before ramping to 95° C. by 0.2° C. increments, stabilizing for 1 second before the Sypro Orange signal was measured. ..



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    Image Search Results


    ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Journal: The Journal of Biological Chemistry

    Article Title: Cyanophycinase is required for heterotrophy in cyanobacteria

    doi: 10.1016/j.jbc.2025.110791

    Figure Lengend Snippet: ArgD binds CphB in both LAH and PAT conditions. A , the relative levels of ArgD and CphB under PAT and LAH in WT and Δ cphB were determined by immunodetection. Whole-cell lysates were separated on SDS-PAGE and blotted onto a PVDF membrane, and the ArgD and CphB proteins were detected using specific antibodies. The Ponceau staining of the membrane is shown for the loading control. Repetitions of the experiment and their statistical analysis are presented in . B , coimmunopurification of f.ArgD with CphB was performed using the same amounts of PAT- or LAH-grown f.argD/ Δ argD cells. The eluates were separated by SDS-PAGE together with the input lysates, including 50% of the lysate from the PAT-grown cells (PAT 50 ). The SYPRO-stained gel was subsequently blotted to a PVDF membrane, which was probed with specific antibodies against CphB and the FLAG tag of ArgD. The control f.ArgD pull-down prepared from the Δ cphB cells is shown in Ref. . CphB, cyanophycinase; f.ArgD, FLAG-tagged variant of ArgD; LAH, light-activated heterotrophic; PAT, photoautotroph; PVDF, polyvinylidene fluoride.

    Article Snippet: The proteins were separated, visualized with SYPRO orange protein dye (Lumiprobe ProteOrange, catalog no.: 40210) and transferred onto a polyvinylidene fluoride membrane (Sigma–Aldrich, Immobilon-P, catalog no.: IPVH00010) that was subsequently incubated with primary anti-CphB and anti-ArgD antibodies ( ).

    Techniques: Immunodetection, SDS Page, Membrane, Staining, Control, FLAG-tag, Variant Assay